CYRIS® 分析平台上进行缺氧分析实验

HYPOXIA ASSAY ON THE CYRIS® ANALYSIS PLATFORM

作者：Christina Heichler & Dr. Peter Wolf（2022）

Abstract：

The following exemplary protocol describes the preparation, run, and results of a hypoxia assay with adherent cells of the cell lines L929, HepG2 and J774A.1 in the CYRIS analysis platform. Oxygen (O2) is essential for the entire organism, but also at the cellular level. The cellular respiration can only function through the provision of sufficient oxygen. In the event of hypoxia, cells switch from aerobic energy production to fermentation to generate energy under anaerobic conditions. The hypoxia assay is utilized to create hypoxic conditions and track the cellular adaption to the reduced oxygen supply by measuring the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of cells. Here we show the procedure with different cell lines (L929, HepG2 and J447A.1). It is possible and may be necessary to adapt the protocol to other cell types, cell numbers, and culture times. It is recommended to read the entire protocol once completely before

文章摘要：

以下案例描述了在 CYRIS 分析平台中使用细胞系 L929、HepG2 和 d J774A.1 的贴壁细胞进行缺氧测定的准备、运行和结果。氧气 (O2) 对整个生物体都是必不可少的，在细胞水平上也是如此。细胞呼吸只能通过提供足够的氧气来发挥作用。在缺氧的情况下，细胞从有氧能量产生转变为发酵以在厌氧条件下产生能量。缺氧测定用于通过测量细胞的耗氧率 (OCR) 和细胞外酸化率 (ECAR) 来创造缺氧条件并跟踪细胞对减少氧气供应的适应。在这里，我们展示了不同细胞系（L929、HepG2 和 J447A.1）的程序。有可能并且可能有必要使案例适应其他细胞类型、细胞数量和培养时间。

点击链接即可查看和下载文章：https://www.cyris.bio/wp-content/uploads/2022/12/20210615-Hypoxia-Application-Note_my.pdf

文章题目、关键词与摘要译文仅用于参考。