CYRIS® 分析平台上的线粒体压力测试

MITOCHONDRIAL STRESS TEST ON THE CYRIS® ANALYSIS PLATFORM

作者：Christina Heichler & Dr. Peter Wolf（2021）

Abstract：

The following exemplary protocol describes the preparation, run, and results of a so-called “mitochondrial stress test” with adherent cells of the cell line HepG2 in the CYRIS analysis platform. The mitochondrial stress test is utilized to create quickly a profile of the key parameters of mitochondrial functions by measuring the oxygen consumption rate (OCR) of cells under the influence of different inhibitors and an uncoupler. Thereby the basal respiration, ATP production, proton leak, maximal respiration, reserve capacity, and non-mitochondrial respiration can be defined. Here we show the procedure with the example of the cell line HepG2 and a faster preparation protocol (seeding and measuring the cells within 6-8 hours). It is possible and may be necessary to adapt the protocol to other cell types, cell numbers, and culture times. It is recommended to read the entire protocol once completely before the first test procedure to avoid problems with time management.

文章摘要：

以下案例描述了在 CYRIS 分析平台中使用细胞系 HepG2 的贴壁细胞进行的所谓“线粒体压力测试”的准备、运行和结果。线粒体压力测试用于通过测量细胞在不同抑制剂和解偶联剂影响下的耗氧率 (OCR)，快速创建线粒体功能关键参数的概况。因此可以定义基础呼吸、ATP 产生、质子泄漏、最大呼吸、储备能力和非线粒体呼吸。

在这里，我们以细胞系 HepG2 和更快的制备方案（在 6-8 小时内播种和测量细胞）为例展示该过程。有可能并且可能有必要使案例适应其他细胞类型、细胞数量和培养时间。

建议在第一次测试程序之前完整阅读整个协议一次，以避免时间管理问题。

点击链接即可查看和下载文章：https://www.cyris.bio/wp-content/uploads/2022/12/20210601-Mito-Stress-Application-Note_my.pdf

文章题目、关键词与摘要译文仅用于参考。