确定 CELL-TAK 的最佳用量以固定悬浮细胞在 CYRIS ®平台上进行进一步分析的实验

DETERMINE THE OPTIMAL AMOUNT OF CELL-TAK TO IMMOBILIZE

SUSPENSION CELLS FOR FURTHER ANALYSIS ON THE CYRIS® PLATFORM

作者：Christina Heichler & Dr. Peter Wolf（2022）

Abstract：

The following protocol describes the preparation, run and results of a preliminary dose-response experiment in the CYRIS® analysis platform to determine the minimum effective density of Cell-Tak to immobilize YAC-1 suspension cells on our metabolic sensor plate for further analysis in the CYRIS® analysis platform. Different blood cells for example lymphocytes or suspension cell lines need to be immobilized for analysis with our automated cell analysis platform CYRIS®. For this application we developed a protocol using Cell-Tak Cell and Tissue Adhesive from Corning® to immobilize suspension cells on the surface of our sensor plates. This special adhesive is based on proteins isolated from the marine mussel Mytilus edulis and is widely used to immobilize suspension cells on different surfaces such as glass or plastic. For optimal immobilization of suspension cells to our sensor plate it is recommended to determine the minimum effective density of Cell-Tak by a preliminary doseresponse experiment as described here.

文章摘要：

以下方案描述了 CYRIS® 分析平台中初步剂量反应实验的准备、运行和结果，以确定 Cell-Tak 的最小有效浓度，将 YAC-1 悬浮细胞固定在CYRIS® 分析平台代谢传感器板上，以便进行进一步分析。

需要固定不同的血细胞，例如淋巴细胞或悬浮细胞系，以便使用自动化细胞分析平台 CYRIS® 进行分析。对于此应用，我们开发了一种方案，使用 Corning® 的 Cell-Tak 细胞和组织粘合剂将悬浮细胞固定在传感器板表面。这种特殊的粘合剂基于从海洋贻贝 Mytilus edulis 中分离出来的蛋白质，广泛用于将悬浮细胞固定在不同的表面上，例如玻璃或塑料。为了将悬浮细胞更好地固定到我们的传感器板上，建议通过此处所述的初步剂量-响应实验来确定 Cell-Tak 的最小有效密度。

点击链接即可查看和下载文章：https://www.cyris.bio/wp-content/uploads/2022/12/20220614-Cell-Tak_Suspension-Cells_Application-Note_my.pdf

文章题目、关键词与摘要译文仅用于参考。