实验归一化后用于活细胞质量归一化的结晶紫测定

PERFORMING A CRYSTAL VIOLET ASSAY FOR LIVING CELL MASS NORMALIZATION FOLLOWING A CYRIS® EXPERIMENT

作者：Christina Heichler & Dr. Peter Wolf（2022）

Abstract：

The following crystal violet (CV) assay can be used to determine living cell mass as an indicator of cytotoxicity or cell proliferation of adherent cell cultures after performance of a CYRIS® experiment. The assay is based on CV staining of living cells attached to the cell culture plate. Adherent cells that have undergone cell death, detach from the cell culture plate and are washed away during the assay. The remaining living cells are stained with CV, which is a cationic triphenylmethane dye that can be used to stain nucleic acids such as DNA. After several washing steps with PBS, CV is released from the stained cells by adding trisodium citrate buffer and can be measured by absorbance at 550 nm. The quantity of CV is directly proportional to the living cell mass attached to the plate. The living cell mass can be used to deduce the level of cytotoxicity or cell proliferation.

文章摘要：

以下结晶紫(CV)试验可用于确定活细胞质量，作为CYRIS®实验后贴壁细胞培养的细胞毒性或细胞增殖的指标。

该检测基于附着在细胞培养板上的活细胞的CV染色。已经历细胞死亡的粘附细胞从细胞培养板分离，并在测定过程中被冲洗掉。剩下的活细胞用CV染色，CV是一种阳离子三苯基甲烷染料，可用于染色DNA等核酸。用PBS洗涤几步后，加入柠檬酸三钠缓冲液从染色细胞中释放CV，并可在550nm处用吸光度测量。

CV的数量与附着在平板上的活细胞质量成正比。活细胞质量可用于推断细胞毒性或细胞增殖水平。

点击链接即可查看和下载文章：https://www.cyris.bio/wp-content/uploads/2022/12/20220407_Crystal-Violet-Staining-Application-Note_my.pdf

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