对巨噬细胞进行实时激活和随后的线粒体压力测试

REAL TIME ACTIVATION AND SUBSEQUENT MITOCHONDRIAL STRESS

TEST OF MACROPHAGES ON THE CYRIS® ANALYSIS PLATFORM

作者：Christina Heichler & Dr. Peter Wolf（2022）

Abstract：

The following exemplary protocol describes the preparation, run, and results of a real time activation and subsequent mitochondrial stress test of adherent cells of the mouse macrophage cell lines J774A.1 in the CYRIS analysis platform. Macrophages, as an important part of the cell-mediated immunity, are activated by external stimuli. Through this, they change their physiological state and also their morphology. By targeted activation, they can be polarized into pro-inflammatory (M1) and immunoregulatory (M2) macrophages. These groups also differ in their metabolic activity. Typical stimuli are pathogen fragments (M1) or cytokines (M2). These can be applied to a macrophage culture and thus force polarization for subsequent investigations. Here we show the polarization of a non-activated (M0) J447A.1 mouse macrophage cell line to M1 and M2 followed by a mitochondrial stress test to determine mitochondrial activity. The complete procedure (polarization and stress test) is performed and analyzed in real time by our CYRIS analysis platform.

文章摘要：

以下示例性方案描述了 CYRIS 分析平台中小鼠巨噬细胞系 J774A.1 贴壁细胞的实时激活和后续线粒体压力测试的准备、运行和结果。

巨噬细胞作为细胞介导免疫的重要组成部分，在受到外界刺激时会被激活。通过这一过程，他们改变了自己的的生理状态也改变了形态。通过靶向激活，它们可以两极分化为促炎症 (M1) 和免疫调节 (M2) 巨噬细胞。这些群体的新陈代谢活动也不同。

典型的刺激是病原体片段 (M1) 或细胞因子 (M2)。这些可以应用于巨噬细胞培养，从而为后续调查强制极化。

在这里，我们展示了非激活 (M0) J447A.1 小鼠巨噬细胞系对 M1 和 M2的极化，随后进行了线粒体压力测试以确定线粒体活性。整个过程程序（极化和压力测试）由 CYRIS 分析平台实时执行和分析。

点击链接即可查看和下载文章：https://www.cyris.bio/wp-content/uploads/2022/12/20220407-Macrophages-stimu-Application-Note_My.pdf

文章题目、关键词与摘要译文仅用于参考。