原动力——不仅仅是膜电位

The protonmotive force - not merely membrane potential

Keywords：BCECF; intramitochondrial pH; matrix pH; ΔpH; mitochondria, mitochondrial membrane potential, ΔΨmt; nigercin; protonmotive force, pmF; reverse electron transfer, RET; safranin; triphenylphosphonium, TPP+; valinomycin 

关键词：BCECF；线粒体内 pH 值；基质 pH 值；ΔpH；线粒体，线粒体膜电位，ΔΨ mt; 尼日利亚菌素；质子动力，pmF；反向电子转移，RET；番红；三苯基鏻，TPP +；缬氨霉素
作者：Komlodi Timea, Tretter Laszlo
出版期刊：《MitoFit Prep》（2022）

Abstract：

The protonmotive force pmF establishes the link between electrical and chemical components of energy transformation and coupling in oxidative phosphorylation in the mitochondrial electron transfer system. The electrical part is corresponding to the mitochondrial membrane potential ΔΨmt and the chemical part is related to the transmembrane pH gradient ΔpH. Although the contribution of ΔpH to pmF is smaller than that of ΔΨmt, ΔpH plays an important role in mitochondrial transport processes and regulation of reactive oxygen species production. Separate measurement of ΔΨmt and ΔpH allows for calculation of pmF. Methods for monitoring ΔΨmt such as fluorescence dyes are generally available, while determination of ΔpH is more challenging.

In this review, we focus on the application of the fluorescence ratiometric method using the acetoxymethyl ester form of 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF/AM) for real-time monitoring of the intramitochondrial pH in isolated mitochondria. Knowing the intra- and extramitochondrial pH allows for calculating the ΔpH. Application of specific ionophores such as nigericin or valinomycin, exerts the possibility to dissect the two components of the pmF in different directions. Furthermore, we tried to summarize those mitochondrial processes, such as production of reactive oxygen species, where the ΔpH has an important role. 

文章摘要：

质子动力pmF在线粒体电子传递系统中的氧化磷酸化中建立能量转换和耦合的电学和化学成分之间的联系。电气部分对应于线粒体膜电位ΔΨ mt，化学部分与跨膜pH梯度ΔpH有关。虽然 ΔpH 对pmF的贡献小于ΔΨ mt，但 ΔpH 在线粒体转运过程和活性氧产生的调节中起着重要作用。单独测量ΔΨ mt和 ΔpH 允许计算pmF。监测ΔΨ mt的方法（例如荧光染料）通常是可用的，而 ΔpH 的测定则更具挑战性。

在这篇综述中，我们重点关注使用 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF/AM) 的乙酰氧基甲基酯形式的荧光比率法实时监测分离线粒体中的线粒体内 pH 值的应用。知道线粒体内和线粒体外的 pH 值可以计算 ΔpH。应用特定的离子载体，如尼日利亚菌素或缬氨霉素，可以在不同方向上剖析pmF的两种成分。此外，我们试图总结那些线粒体过程，例如活性氧的产生，其中 ΔpH 具有重要作用。

点击链接即可查看和下载文章：https://wiki.oroboros.at/images/7/7f/Komlodi_2022_MitoFit_Preprints.pdf

文章题目、关键词与摘要译文仅用于参考。