六周的高强度循环训练可减少具有 2 型糖尿病危险因素的肥胖成年人的 H2O2 排放并增加抗氧化蛋白水平

Six weeks of high intensity cycle training reduces H2O2 emission and increasesantioxidant protein

levels in obese adults with risk factors for type 2 diabetes.

Keywords：Antioxidants, HIT, Oxidative stress, Skeletal muscle.

关键词：抗氧化剂，HIT，氧化应激，骨骼肌

哺乳动物：人
作者：Flensted-Jensen Mathias, Gram Martin, Dela Flemming, Helge Joern Wulff, Larsen Steen

出版期刊：《Free Radical Biology and Medicine》（2021年7月14日）

Abstract：

Obesity has been associated with increased production of reactive oxygen species (ROS), which may be involved in the development of cardiovascular disease and type 2 diabetes (T2D). Endurance exercise lowers ROS production and increases antioxidant capacity in muscle cells, but it is currently unknown whether high intensity interval training (HIT) elicits the same effects. Twelve sedentary obese subjects at risk of developing T2D took part in a six-week intervention, performing three HIT sessions per week (five 1-min sets of high-intensity cycling (125% of VO2peak), with 90 s recovery in between sets). Muscle biopsies were obtained for assessment of ROS production (H2O2 emission), mitochondrial respiratory capacity, and antioxidant protein levels before and after the intervention. H2O2 emission decreased 60.4% after the intervention (Succinate 3 mmol･l-1), concurrent with a 35.1% increase in protein levels of the antioxidant manganese superoxide dismutase (MnSOD) and a trend towards increased levels of the antioxidant catalase (p = 0.06, 72.9%). These findings were accompanied by a 19% increased mitochondrial respiratory capacity (CI + II), a 6.9% increased VO2peak and a 1.7% lower body fat percentage. These effects were achieved after just 15 min of high-intensity work and 40 min of total time spent per week. Overall, this suggests that a relatively small amount of HIT is sufficient to induce beneficial effects on ROS production and antioxidant status in muscle cells, which may lower oxidative stress and potentially protect against the development of cardiovascular disease. 

文章摘要：

肥胖与活性氧 (ROS) 的产生增加有关，这可能与心血管疾病和 2 型糖尿病 (T2D) 的发展有关。耐力运动会降低肌肉细胞中 ROS 的产生并增加抗氧化能力，但目前尚不清楚高强度间歇训练 (HIT) 是否会产生相同的效果。 12 名有患 T2D 风险的久坐肥胖受试者参加了为期 6 周的干预，每周进行 3 次 HIT 训练（5 组 1 分钟的高强度循环（125% 的 VO2peak），组间恢复 90 秒） .获得肌肉活检以评估干预前后的 ROS 产生（H2O2 排放）、线粒体呼吸能力和抗氧化蛋白水平。干预后 H2O2 排放量减少了 60.4%（琥珀酸盐 3 mmol･l-1），同时抗氧化锰超氧化物歧化酶 (MnSOD) 的蛋白质水平增加了 35.1%，并且抗氧化过氧化氢酶水平呈上升趋势（p = 0.06 , 72.9%)。这些发现伴随着线粒体呼吸能力（CI + II）增加 19%、VO2peak 增加 6.9% 和体脂百分比降低 1.7%。这些效果是在 15 分钟的高强度工作和每周花费 40 分钟的总时间后实现的。总体而言，这表明相对少量的 HIT 足以诱导对肌肉细胞中 ROS 产生和抗氧化状态的有益影响，这可能会降低氧化应激并可能预防心血管疾病的发展。

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