C. crangon 和 P. serratus 中不存在 Ca2+ 诱导的线粒体通透性转变，但存在 Bongkrekate 敏感核苷酸交换

Absence of Ca2+-Induced Mitochondrial Permeability Transition but Presence

of Bongkrekate-Sensitive Nucleotide Exchange in C. crangon and P. serratus

Keywords：Ca²⁺; mitochondrial permeability
关键词： Ca²⁺ ；线粒体通透性
作者：Konrad C, Kiss G, Torocsik B, Adam-Vizi V, Chinopoulos C
出版期刊：《PLoS One》 2012/6/29

Abstract：

Mitochondria from the embryos of brine shrimp (Artemia franciscana) do not undergo Ca²⁺-induced permeability transition in the presence of a profound Ca²⁺ uptake capacity. Furthermore, this crustacean is the only organism known to exhibit bongkrekate-insensitive mitochondrial adenine nucleotide exchange, prompting the conjecture that refractoriness to bongkrekate and absence of Ca²⁺-induced permeability transition are somehow related phenomena. Here we report that mitochondria isolated from two other crustaceans, brown shrimp (Crangon crangon) and common prawn (Palaemon serratus) exhibited bongkrekate-sensitive mitochondrial adenine nucleotide transport, but lacked a Ca²⁺-induced permeability transition. Ca²⁺ uptake capacity was robust in the absence of adenine nucleotides in both crustaceans, unaffected by either bongkrekate or cyclosporin A. Transmission electron microscopy images of Ca²⁺-loaded mitochondria showed needle-like formations of electron-dense material strikingly similar to those observed in mitochondria from the hepatopancreas of blue crab (Callinectes sapidus) and the embryos of Artemia franciscana. Alignment analysis of the partial coding sequences of the adenine nucleotide translocase (ANT) expressed in Crangon crangon and Palaemon serratus versus the complete sequence expressed in Artemia franciscana reappraised the possibility of the 208-214 amino acid region for conferring sensitivity to bongkrekate. However, our findings suggest that the ability to undergo Ca²⁺-induced mitochondrial permeability transition and the sensitivity of adenine nucleotide translocase to bongkrekate are not necessarily related phenomena.

文章摘要：

来自卤水虾（Artemia franciscana）胚胎的线粒体在 Ca²⁺ 吸收能力强的情况下不会经历 Ca²⁺ 诱导的渗透性转变。此外，这种甲壳类动物是已知的唯一一种表现出对 bongkrekate 不敏感的线粒体腺嘌呤核苷酸交换的生物，这促使人们推测，对 bongkrekate 的耐火性和缺乏 Ca²⁺ 诱导的渗透性转变是某种相关的现象。在这里，我们报告从其他两种甲壳类动物、褐虾 (Crangon crangon) 和普通虾 (Palaemon serratus) 中分离出的线粒体表现出对 bongkrekate 敏感的线粒体腺嘌呤核苷酸转运，但缺乏 Ca²⁺ 诱导的渗透性转变。在两种甲壳类动物中，在没有腺嘌呤核苷酸的情况下，Ca²⁺ 摄取能力很强，不受 bongkrekate 或环孢菌素 A 的影响。负载 Ca²⁺ 的线粒体的透射电子显微镜图像显示，电子致密物质的针状结构与从线粒体中观察到的非常相似。蓝蟹（Callinectes sapidus）的肝胰腺和卤虫的胚胎。在 Crangon crangon 和 Palaemon serratus 中表达的腺嘌呤核苷酸转位酶 (ANT) 的部分编码序列与在 Artemia franciscana 中表达的完整序列的比对分析重新评估了 208-214 氨基酸区域赋予对 bongkrekate 敏感性的可能性。然而，我们的研究结果表明，经历 Ca²⁺ 诱导的线粒体通透性转变的能力和腺嘌呤核苷酸转位酶对 bongkrekate 的敏感性不一定是相关的现象。

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