间充质干细胞与水溶性姜黄素的启动允许保持其间充质特性以用于基于细胞的治疗开发

Priming of mesenchymal stem cells with a hydrosoluble form of curcumin allows keeping their mesenchymal properties for cell-based therapy development

Keywords：NDS27, Curcumin, Cyclodextrin, Equine MSCs, mdMSC, Mesenchymal stem cells, Mitochondria

关键词：NDS27、姜黄素、环糊精、马MSCs、mdMSC、间充质干细胞、线粒体

哺乳动物：马

作者：Margaux Colin,Lola Dechêne,Justine Ceusters,Ariane Niesten,Catherine Demazy,Laurence Lagneaux,Karim Zouaoui Boudjeltia,Thierry Franck,Pierre Van Antwerpen,Patricia Renard,Véronique Mathieu,Didier Serteyn

出版期刊：《JOURNAL OF CELLULAR AND MOLECULAR MEDICINE》 2021/3/26

Abstract：

Mesenchymal stem cells are increasingly studied for their use as drug-carrier in addition to their intrinsic potential for regenerative medicine. They could be used to transport molecules with a poor bioavailability such as curcumin in order to improve their clinical usage. This natural polyphenol, well-known for its antioxidant and anti-inflammatory properties, has a poor solubility that limits its clinical potential. For this purpose, the use of NDS27, a curcumin salt complexed with hydroxypropyl-beta-cyclodextrin (HPβCD), displaying an increased solubility in aqueous solution, is preferred. This study aims to evaluate the uptake of NDS27 into skeletal muscle-derived mesenchymal stem cells (mdMSCs) and the effects of such uptake onto their mesenchymal properties. It appeared that the uptake of NDS27 into mdMSCs is concentration-dependent and not time-dependent. The use of a concentration of 7 µmol/L which does not affect the viability and proliferation also allows preservation of their adhesion, invasion and T cell immunomodulatory abilities.

文章摘要：

间充质干细胞除了具有再生医学的内在潜力外，还越来越多地被研究用作药物载体。它们可用于运输姜黄素等生物利用度差的分子，以改善其临床应用。这种天然多酚以其抗氧化和抗炎特性而闻名，但溶解度差，限制了其临床潜力。为此目的，优选使用 NDS27，一种与羟丙基-β-环糊精 (HPβCD) 复合的姜黄素盐，在水溶液中显示出增加的溶解度。本研究旨在评估 NDS27 对骨骼肌间充质干细胞 (mdMSCs) 的摄取以及这种摄取对其间充质特性的影响。似乎 NDS27 摄入 mdMSCs 是浓度依赖性的，而不是时间依赖性的。使用不影响活力和增殖的 7 µmol/L 浓度还可以保留它们的粘附、侵袭和 T 细胞免疫调节能力。

点击链接即可查看和下载文章：https://onlinelibrary.wiley.com/doi/10.1111/jcmm.16403

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