高分辨率呼吸测量法在冷冻保存和移植卵巢组织中的代谢活性

Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry

Keywords：Energy metabolism, Infertility

关键词：能量代谢，不孕不育

组织：生殖器
作者：Rodrigues AQ, Picolo VL, Goulart JT, Silva IMG, Ribeiro RB, Aguiar BA, Ferreira YB, Oliveira DM, Lucci CM, de Bem AF, Paulini F 
出版期刊：《Scientific Reports》 2021/11/2

Abstract：

Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function.

文章简介：

卵巢组织冷冻保存后移植代表了恢复卵巢功能和生育能力的策略。冷冻保存和解冻过程产生的压力会导致线粒体结构和功能的改变和/或损坏。高分辨率呼​​吸测量和组织学分析用于评估冷冻保存和移植对卵巢组织的影响。进行了四种不同的条件：新鲜非移植组织、新鲜移植组织、冷冻保存的非移植组织和冷冻保存的移植组织。所有组都能够对底物-解偶联剂-抑制剂方案作出反应。我们发现接受冷冻保存和/或移植的半卵巢的一般耗氧量显着降低。冷冻保存对线粒体代谢的影响不如移植的影响强烈，因为移植影响了所有的线粒体状态。共分析了 2644 个卵泡。其中，2198 例被归类为形态正常。与冷冻保存非移植组和新鲜移植组相比，冷冻保存移植组形态正常卵泡的百分比显着降低（p值 < 0.05）。尽管卵泡活力和线粒体活性降低，但冷冻保存然后移植卵巢组织被证明对于恢复卵巢功能是可行的。

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